KMT2D Knockout cell line (HEK293)

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location： Home > Products > KO Cell Lines > KMT2D Knockout cell line (HEK293)

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KMT2D Knockout cell line (HEK293) Order

KMT2D Knockout cell line (HEK293)
Catalog#
YKO-HS12193
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KMT2D Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KMT2D Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
KMT2D
Gene ID
8085
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript KMT2D-201)
Validation: PCR+Sanger Sequencing
KMT2D Knockout cell line (HEK293) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293		 STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KMT2D gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KMT2D gene knockout KMT2D Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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